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Hay is the foundation of the majority of horse, donkey and mule diets - yet it rarely includes a nutritional analysis. Most of us would never consider purchasing any type of bagged feed or supplement without a label/nutritional analysis. Testing your hay is the only way to know the nutritional values - allowing you to compensate for deficiencies, imbalances (ratios) and possible excessive components.
A balanced core diet is necessary to achieve optimum health for your horse or herd. Read on to learn how to test your hay, where to send the sample and how to calculate NSCs using the results. Related links are provided to guide you through the process. 
Having your hay analyzed provides crucial information about levels of protein, minerals (including ratios), digestible and indigestible fiber and sugar and starch levels. The results reveal what may be deficient or excessive in the diet, including non-structural carbohydrates (NSCs). If you have a metabolically challenged horse, donkey or mule and are feeding untested hay, this simple process can also alleviate the task of soaking hay if the NSCs are 10% or below.
Items You Will Need to Test Hay:
Hay probe & plunger to empty the contents.
How to Take a Sample in 5 Steps
1. Wash a bucket with soap and water, rinse well and dry. A clean plastic bucket (not metal) ensures a minimum amount of contaminates (dirt, dust or other feeds that the bucket has been used for) in the sample.
2. Be prepared to collect a minimum of 12-16 samples throughout the load of hay. (You need enough hay to fill two quart size Ziplock bags.) Most probes will accommodate a sample from 2 bales of hay. IMPORTANT TIP: Insert the probe at the end of the bale (not from the side) a minimum of 12”-15”. 
Insert the probe at the end of the bales.
3. After you have cored 2 bales of hay, disconnect the probe from your drill and empty the contents into the clean plastic bucket. Repeat, collecting samples throughout the entire load until you have probed enough bales to accurately represent the entire load of hay. If you have over 100 bales, a good rule of thumb is to probe 15% of your hay.
4. When you have finished collecting your samples, wash and dry your hands and blend all samples together in the clean bucket. 
Blend the samples well with clean hands.
5. Prepare two Ziploc bags by writing your name, date and type of hay on the baggies using a Sharpie® permanent marker. It’s important to identify the sample should the Ziploc bag and order form become separated at the lab. You will mail one bag to the lab and keep one in case it is lost in the mail or the test results warrant a retest. This prevents you from having to repeat the process of collecting samples for a second time from the same load of hay.
It’s important to identify the sample should the bag and order form become separated at the lab.
Where to Mail Your Sample
My preference is Equi-Analytical Labs as opposed to local dairy farm labs. Equi-Analytical offers a variety of affordable comprehensive analytical service packages. The tests are available to be reported in English (Mcal/lb.) as opposed to Metric (Mcal/kg).
The test I order is the (601) Equi-Tech – It includes moisture, dry matter, digestible energy, crude protein, estimated lysine, acid detergent fiber, neutral detergent fiber, lignin, Ethanol Soluble Carbohydrates (ESC), Water Soluble Carbohydrates (WSC), starch, non-fiber carbohydrates (NFC), fat, ash, calcium, phosphorus, magnesium, potassium, sodium, iron, zinc, copper, manganese, molybdenum. The cost is only $28.00. For an additional $11.00, I add nitrates to the test. Find out why you should test for levels of nitrates, how they deprive your horse of oxygen and how to convert/interpret your test results. If you are testing your hay exclusively to assess NSC's the (600) Fast Track is extremely economical ($18.00) and includes moisture, dry matter, digestible energy, crude protein, acid detergent fiber, neutral detergent fiber, Ethanol Soluble Carbohydrates (ESC), Water Soluble Carbohydrates (WSC), starch, non-fiber carbohydrates (NFC), calcium, and phosphorus. This is a wise investment if you are currently soaking untested hay. Complete the online sample submission form and print. In an envelope, include your sample submission form and hay sample and mail it to: Equi-Analytical Laboratory Services 730 Warren Road Ithaca, New York 14850 
Receiving Your Results
Results are emailed once completed. I typically receive mine within 2-3 days once it has been delivered to the lab.
Interpreting Your Results
To interpret your analysis and balance the diet to your hay and supplements, your options are to:
*Dr. Kellon's online course provides the most accurate comprehensive methods to balance the diet by providing optimum ratios of minerals in addition to NRC nutritional requirements. How to Calculate NSCs (NonStructual Carbohydrates)
To calculate NSCs (nonstructural carbohydrates) once you receive the results:
Add the Starch % + ESC % = NSC%
or Add the Starch % + WSC % = NSC% What Are the Definitions of Starch, ESC and WSC?
Why Do Vets and Nutritionists Quibble over What Percentages to Use As the NSC Sum?
“The nutritional relevancy of ESC vs WSC has not been researched due to the unavailability of fructan from grass in a pure form. All the studies implicating fructan in laminitis, insulin response, and gut pH have been done on fructan sourced from broad leaf weeds. It is very different, being shorter and more prone to rapid fermentation. Hence the uncertainty of the effects of grass fructan on horses and whether ESC or WSC is more relevant. For sure, fructans from broad leaf weeds are bad.” Katy Watts, Founder of Safergrass.org. Learn more about Sugar Content of Pasture & Hay: Q & A with Katy Watts, Founder of Safergrass.org Testing Your Hay is Easy & Affordable
Testing your hay is the best insurance policy to ensure you are meeting your horse's or herd's daily nutritional requirements. For as little as $18 - it’s affordable and practical.
Whether you have an entire year's supply of hay in your barn - or enough to last 3 months - it’s a great investment with the added benefit of alleviating the task of soaking hay if your herd is metabolically challenged and the NSC results are 10% or lower. Don’t be intimidated by the process - it is quick and easy. By the second time you take samples, you’ll be a pro! Helpful How To Resources
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About the Author:
Monique Warren invented the Hay Pillow® slow feeder and is the owner of Hay Pillow Inc. Warren has been an equine guardian for over forty years and slow-feed advocate for over 10 years. She contributes equine nutrition and digestive and hoof health articles to publications such as The Naturally Healthy Horse, Natural Horse Magazine, Nicker News, Horse Back Magazine, The Horse's Hoof, and Miniature Horse World Magazine. Equine nutrition and horses feet are her passions. She resides in Southern California.
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